2026-04-21T19:19:49Z https://datacatalogue.cessda.eu/oai-pmh/v0/oai

e32964b772364957326becda591c80b045332ba0339bba50756758b5808542f3 2025-06-17T03:26:05Z language:svopenaire_data

RNA-sekvenseringsdata från patienter med hjärtsjukdom och kontroller Swedish National Data ServiceSvensk nationell datatjänst Landing page RNA-sekvenseringsdata från patienter med hjärtsjukdom och kontroller RNA sequencing data from patients with heart disease and controls 2023-80-1-1https://doi.org/10.5878/e48r-gn02 Rotter Sopasakis, Victoria Rotter Sopasakis, Victoria Mattsson Hultén, Lillemor Mattsson Hultén, Lillemor Swedish National Data ServiceSvensk nationell datatjänst 2023-04-17 Landing page Heart DiseasesHjärtsjukdomarAortic ValveAortaklaffCoronary Artery BypassKoronar-bypassCadherinsKadherinerHeart Valve Prosthesis ImplantationHjärtklaffprotesimplantationRNA, Long NoncodingLång-ickekodande RNACadherin Related ProteinsKadherinrelaterade proteinerCardiovascular DiseasesHjärt-kärlsjukdomarCardiac Surgical ProceduresHjärtkirurgiska teknikerHeart ValvesHjärtklaffarMyocardial RevascularizationMyokardrevaskulariseringCell Adhesion MoleculesCelladhesionsmolekylerProsthesis ImplantationProtesimplantationRNA, UntranslatednpcRNAVascular GraftingKärltransplantationAntigens, SurfaceYtantigenerHeartHjärtaMembrane GlycoproteinsMembranglykoproteinerRNARNACardiovascular Surgical ProceduresHjärt-kärlkirurgiska ingreppSurgical Procedures, OperativeKirurgiska ingreppVascular Surgical ProceduresKärlkirurgiThoracic Surgical ProceduresToraxkirurgiska teknikerCardiovascular SystemHjärt-kärlsystemetAntigensAntigenerGlycoproteinsGlykoproteinerMembrane ProteinsMembranproteinerNucleic AcidsNukleinsyrorProteinsProteinerBiological FactorsBiologiska faktorerGlycoconjugatesGlykokonjugaterNucleic Acids, Nucleotides, and NucleosidesNukleinsyror, nukleotider och nukleosiderCarbohydratesKolhydraterAmino Acids, Peptides, and ProteinsAminosyror, peptider och proteiner Biochemistry and Molecular BiologyBiokemi och molekylärbiologiGeneticsGenetikBioinformatics and Systems BiologyBioinformatik och systembiologiBasic MedicineMedicinska och farmaceutiska grundvetenskaperMedical GeneticsMedicinsk genetikCell and Molecular BiologyCell- och molekylärbiologiCardiac and Cardiovascular SystemsKardiologiBiological SciencesBiologiMedical and Health SciencesMedicin och hälsovetenskapClinical MedicineKlinisk medicinNatural SciencesNaturvetenskap RNA sequencing analysis of atrial cardiac tissue from patients undergoing coronary artery bypass grafting (CABG) or aortic valve replacement (AVR) was performed and compared with atrial cardiac tissue from organ donors and purchased human atrial cardiac RNA. RNA sequencing analysis was performed at the Genomics Core Facility at University of Gothenburg, Sweden. All samples (ten controls, ten CABG patients and ten AVR patients) were quality checked by the RNA integrity number (RIN) using Tapestation 2200 RNA screenTape (Agilent Technologies, Santa Clara, CA) and RNA concentration was measured by NanoDrop (Thermo Fisher, Waltham, MA). RIN values ranged between 6.6 and 9.0 for all samples. TruSeq Total Stranded RNA kit with RiboZero (Gold) Sample Preparation Guide (15,031,048 Rev. E) was used for RNA sample preparation (Illumina, San Diego, CA). A total of 10 μl (~1 μg) RNA from each sample was used for library preparation. Directly after depletion, a cleanup step was performed using 110 ul of the RNAClean XP beads (Beckman Coulter, USA) for each sample. The fragmentation step was performed for 8 min. 12 PCR cycles were run for all samples. Libraries were quantified and normalized with Qubit DNA HS Assay kit (Life Technologies, Carlsbad, CA) and fragment size determined by Tapestation 2200 (Agilent Technologies, Santa Clara, CA). The libraries were pooled together by using the Illumina protocol for pooling and sequenced with NovaSeq 6000 S1 (Illumina, San Diego, CA) for the read length of 2 × 100 bp.Data i denna studie kommer från RNA-sekvenseringsanalys av förmaksvävnad från humana hjärtan från patienter som genomgick coronary artery bypass grafting (CABG) eller aortic valve replacement (AVR) samt förmaksvävnad från organdonatorer (kontroller) och inköpt humant RNA från förmaksvävnad (kontroller). SwedenSverige Individual/PatientIndivid/patient/person Non-probability: AvailabilityIcke-sannolikhetsurval: tillgänglighetsurvalNon-probabilityIcke-sannolikhetsurval Access to data through SND. Access to data is restricted.Åtkomst till data via SND. Tillgång till data är begränsad. https://snd.gu.se 2023-80-1 2023-04-17T15:31:22Z ddi:codebook:2_5